Anti-Connexin-37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

Rat Connexin 37 is A 333 aa gap junction protein with a predicted MW. Of ~37kD. It is prominently expressed in lung (see reviews in refs. 1). A 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of rat Cx37 (2) was used to generate anti-Mouse Cx37. Peptide was coupled to KLH (carrier protein) Via the N-terminal Cysteine and antibodies generated in rabbits. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique. ELISA (1:10K-100K using 50-100ng control peptide/well. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.