Anti-Connexin-31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

Connexin 31 (Cx31) is a 270 amino acid gap junction protein in mouse and rat with a predicted molecular weight of ~31kD. Connexin 31 is encoded by the gene Gjb3, and is expressed in a complex pattern throughout mouse development. During early mouse development, Cx31 is present in both the inner cell mass and the trophectoderm, but it is restricted to cells derived from the trophectoderm lineage after implantation. In adult female rodents, Cx31 is expressed in the granular layer of the placenta, and the lack of Cx31 leads to transient dysmorphogenesis affecting embryonic survival. In adult male rodents, Cx31 is expressed in the seminiferous epithelium of testis and may play a role in cell-cell communication during spermatogenensis. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1-3ug/ml, Western Blot: 1-3ug/ml, Immunohistochemistry: 1-3ug/ml on fixed and paraformaldehyde-perfued tissues, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.