Anti-COMP (Cartilage Oligomeric Matrix Protein, Thrombospondin-5, TSP5)

Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, Immunohistochemistry (Paraffin): 5-20ug/ml, Immunocytochemistry: 5-20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control Included: 139591A: COMP, Positive Control, Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)., DAB: 10ul/well, ECL: 5ul/well, Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.