Anti-Clusterin (Aging-associated Gene 4 Protein, Apolipoprotein J, Apo-J, Complement Cytolysis Inhib

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40, is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development. Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain. Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized A and B chains which are secreted as an 80kD N-glycosylated disulfide-linked heterodimer. Mature human Clusterin shares 77% aa sequence identity with mouse and rat Clusterin. High ug/ml concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin. In human, an alternately spliced 50kD isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular. This molecule is neither glycosylated nor cleaved into A and B chains. In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NFKB activation. Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis. This function contrasts with the cytoprotective effect of secreted Clusterin. During colon cancer tumor progression there is a downregulation of the intracellular form and an upregulation of the glycosylated secreted form. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.