Anti-Citrullinated Vimentin (Clone 12G11)

Vimentin is a cytoskeleton intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2, Cay-10785) under high calcium concentrations, which can occur during macrophage apoptosis. Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs). Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for ACPAs. Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively. Cayman's Citrullinated Vimentin Monoclonal Antibody (Clone 12G11) can be used for ELISA, immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes citrullinated vimentin at approximately 54 kDa from human samples.Immunogen: Synthetic peptide from human vimentin with citrullines at 144 and 146 (G146R mutation). Clone Designation: 12G11. Formulation: (Request formulation change), 100 µg of protein G-purified antibody. Storage Buffer: PBS, pH 7.2, with 0.02% sodium azide (avoid freeze/thaw cycles). Host: Mouse. Isotype: IgG1. Applications: ELISA, WB, and IP. Cross Reactivity: (+) Citrullinated vimentin, (-) Unmodified protein. UniProt Accession #: P08670.