Anti-Chitotriosidase (Chitinase 1, CHIT-1)

Human CHIT-1 (chitotriosidase-1, also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi and nematodes. Mature CHIT-1 is 435 amino acids (aa) in length, and contains a catalytic domain (aa22-372) followed by a chitin-binding region (aa373-466). Very high levels of CHIT1 are found in the plasma of patients with Gaucher's disease type I. Mature human CHIT-1 is 79% aa identical to mouse CHIT-1. Applications: Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well., Immunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium. , Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/ml (IC 50) under conditions in which the enzyme was present at 20ng/ml and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.