Anti-CDw150 (CD150, Signaling Lymphocytic Activation Molecule (SLAM)

Signaling lymphocytic activation molecule (SLAM, SLAMF1, CD150) was the first identified of , a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic , adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic , stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70kD , glycoprotein contains a 216aa extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20aa TM sequence and a 76aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.