Anti-CDK4 (Cyclin-dependent Kinase 4, Cell Division Protein Kinase 4, CMM3, Cyclin-dependent Kinase

CDK4 (cyclin-dependent kinase 4) is a 34kD member of the CMGC Ser/Thr protein kinase family of enzymes. Within the cell, pRb/retinoblastoma protein is normally unphosphorylated and suppresses cell growth at the G1 stage of the cell cycle. When mitosis is required, CDK4 is phosphorylated, forms a complex with cyclin D1, 2 or 3, and phosphorylates pRb/retinoblastoma protein. This inactivates pRb and cells initiate DNA synthesis. Human CDK4 is 303aa in length. It contains one protein kinase domain (aa6-295) and an activating phosphorylation site at Thr172. There are three potential variants, one that shows an alternate start site at Met75, a second that shows a twoaa substitution for aa89-211, and a third that shows a 23aa substitution for aa89-303. Full-length human and mouse CDK4 are 95% aa identical. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.