Anti-CDCP1 (Cub Domain Containing Protein1, SIMA135)

CDCP1 is a novel, 135kD cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa30-667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa's 270-300. This generates an 80kD TM protein that may be missing the N-terminal CUB domain (aa221-350). Over aa's 25-667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml. Recombinant mouse CDCP1 , Immunohistochemistry: 5-15ug/ml. Immersion fixed frozen sections of mouse embryo (E11-13), Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.