Anti-CD59 (MACIF, MIRL, Protectin) (PE)

Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (~80ul/test), Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x 10e5 cultured HPB-MLT cells were washed and preincubated ~5 minutes with 20ul of 250mg/ml human IgG (to block nonspecific binding). , 2. After which they were incubated 45 minutes on ice with 80ul of C2413-21R at a dilution of 1:50. , 3. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. , 4. Cells stained positive with a mean shift of 2.81 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. , 5. Binding was 92% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2413-21N. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.