Anti-CD56 (NCAM-1, Neural Cell Adhesion Molecule) (Biotin)

Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): acetone fixation, Flow Cytometry: 10ug/ml will label 10e5 cells, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral white blood cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80ul of anti-CD56/Biotin at a concentration of 10ug/ml. , 2. Cells were washed twice and incubated with 2o reagent Streptavidin (PE) (Catalog #S7973-88P)., 3. After which they were washed three times, fixed and analyzed using by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 (Biotin) negative control at a similar concentration. , 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD56 antibody (Catalog #C2410-55A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.