Anti-CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Ly

CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1 or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing and in tumor metastasis., This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA or CD2 (1). This antibody enhances proliferation of PBL stimulated with CD2 (1). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 2ug Ab/mg protein lysate, Use Protein A, Native verified, Western Blot: 1-2ug/ml for 2 hrs. at RT, Immunohistochemistry: 1-2ug/ml for 30min. at RT, Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min., Optimal dilutions to be determined by the researcher. Positive Control: HUVEC or HeLa Cells. Tonsil or esophageal carcinoma, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.