Anti-CD41 (Integrin alpha 2b, GP2B, GPIIb, GTA, HPA3, ITGA2b)

Integrin alpha2b (ITGA2b), also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114kD and 22kD) during transit through the Golgi. Integrin alpha2b associates with Integrin beta3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. Integrin alpha2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, human Integrin alpha2b shares 80% and 78% amino acid sequence identity with mouse and rat Integrin alpha2b, respectively. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells, Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.