Anti-CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane M

CD10 also known as common acute lymphoblastic leukemia antigen (CALLA), is a 100kD protein having neutral metalloendopeptidase activity and inactivates a variety of biologically active peptides. CD10 is a type II cell surface glycoprotein generally expresses mostly on acute lymphoblastic leukemias (ALL). In normal healthy volunteers precursors of B and T cells and also on granulocytes also expressed CD10. CD10 plays important role in the development process of B cell in germinal centers. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is enough to stain 1x10e6 PBMCs or 100ul whole blood sample followed by a second staining with a fluorescent conjugated streptavidin, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.