Anti-Calponin 1 (Calponin H1, CNN1, Sm-Calp, SMCC, Calponin-1, Calcium Calmodulin-binding Troponin T

CNN­1 (calponin­1 [calcium and calmodulin­binding troponin T­like protein], also calponin basic, CaP and calponin H1) is a 32­36kD cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth muscle­like cells such as myoepithalium and myofibroblasts in the adult. CNN­1 interacts with F­actin in a phosphorylation­dependent manner. When nonphosphorylated, CNN­1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN­1 expression inhibits cell motility and the formation of podosomes. Human CNN­1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa30­127), and three consecutive calponin­like repeats (aa164­268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Full­length human CNN­1 shares 97% aa sequence identity with mouse CNN­1. Applications: Suitable for use in ELISA, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml, Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.