Anti-Calcium and Integrin Binding Protein 1 (SIP2-28, Calmyrin)

CIB1 (Calcium and integrin binding 1) is regulatory protein with 50% homology to calmodulin and calcineurin B, that encodes a member of the calcium-binding protein family. The specific function of this protein has not yet been determined, however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:2,000, Western Blot: 1:1000-1:2,000, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CIB1 protein. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.