Anti-Cadherin R (Cadherin 4, Cadherin-4, Retinal Cadherin, CDH4, R-CAD, R-cadherin)

Cadherin-4 (also Retinal/R-Cadherin) is a 120kD (100kD predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic B-cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893aa in length. It contains a 146aa propeptide aa21-166 coupled to a 746aa type I transmembrane mature protein aa 167-913. The mature region possesses a 565aa extracellular domain aa167-731 plus a 160aa cytoplasmic region aa753-913. Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain aa167-721. There is one potential splice form that shows a 44aa substitution for aa291-913. Over aa21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.