Anti-b-Amyloid, Oligomer A11

Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. In disease state oligomerization, extensive amyloid oligomerization creates plaques in neural tissue that correlates with Alzheimer's symptomology. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: Direct, Western Blot: 1:500 detected Amyloid Oligomer on 10ug of mouse brain lysates., Immunoprecipitation: 1:1000. Suggested cell lysis buffer is RIPA. Suggested capture agent is magnetic beads (Dynabeads). Known co-precipitatiing polypeptide: Amyloid beta, alpha synuclein oligomers., Immunofluorescence: 1:200 diluted 1:1 in a mixture of blocking solution and PBS (refer to protocol), Immunohistochemistry: 1:1000-1:10,000, Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.,