Anti-Apolipoprotein E (Apo E)

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. Apolipoprotein E is a type of lipoprotein. Apolipoprotein E is associated with several cardiovascular disorders. There are three isoforms (slightly different forms) of the ApoE lipoprotein. These three isoforms are known as ApoE2, E3 and E4. ApoE2 seems to be one of several genetic factors that plays a part in increased risk of heart attacks and strokes and ApoE4 is a risk factor for coronary artery disease. As well, ApoE4 has been been suggested to play a role in type 2 Alzheimer disease. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug/10e6 cells, ELISA: 1:100-1:2000, Western Blot: 2ug/ml. A band observed at ~36kD, Immunoprecipitation: 1:10-1:500, Immunofluorescence (IC): 1:200, Immunohistochemistry: 1:50-1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.