Anti-Apolipoprotein C-II (Apo C-II) (Biotin)

Apolipoprotein C2 (ApoC2/II) is a 8-9kD, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43-51) and an enzyme interaction site (aa 55-78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23-28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/mL, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.