Anti-Angiotensin II (Hypertensin II, Ang II)

Ang II plays an important role in regulating natriuretic peptide gene expression during cardiac overload. ANP and BNP gene expression in the rat heart is modulated by the adrenal factors during Ang II-stimulated hemodynamic stress and the AT1 receptor antagonism in NO-deficient hypertension. Moreover, ventricular BNP gene expression in Ang II-induced hypertension in vivo is controlled by post-transcriptional mechanisms and GATA elements. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:250-1:16,000, Immunohistochemistry: Frozen and paraffin, PAP, ABC: 1:250-1:16,000, LAB-SA: 1:250-1:16,000, Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.