Anti-AMP Activated Protein Kinase gamma2 (AMPKg2)

AMPK plays a key role in the regulation of energy homeostasis. It acts as a metabolic stress-inducing protein kinase that switches off biosynthetic pathways when cellular ATP levels are low and when 5`-AMP rises in response to fuel limitation. AMPK is activated by high AMP and low ATP via a mechanism that involves allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase. May increase the rate of glucose oxidation in muscle by increasing the rate of glucose transport. Shows homology to yeast protein kinase-encoding gene (snf1) regulating carbohydrate metabolism. Human AMPKg2 is a 569 aa protein, which contains unique N-terminal domains and participate in binding of AMP within the AMPK complex. AMPK is a heterotrimeric complex composed of a catalytic subunit and two regulatory subunits. Three isoforms of the gamma subunit exist in the brain with gamma 2 and gamma 3 present, which is distinct from the rat liver gamma 1 isoform. The noncatalytic subunits of AMPK are related to proteins that interact with snf1p, the beta subunit is related to the SIP1/SIP2/ GAL83 family of transcription regulators and the gamma subunit to SNF4.3 Mutations in AMPKgamma 2 can cause familial hypertrophic cardiomyopathy (HCM), which provides evidence for the central role of energy compromise in disease pathogenesis. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.