Anti-Adipophilin (ADFP, Adipose Differentiation-related Protein)

Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines, inducible by etomoxir. Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. New specific marker for liposarcoma. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applicatons not tested. Recommended Dilutions: Western Blot: 1:2000, Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100, Incubate for 1hr at RT or overnight at 4°C. , Optimal dilutions to be determined by the reseracher. Immunohistochemistry Positive Controls: Glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertolis cells of the testis, fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver (adipocytes are negative), lipid storing CD68-positive macrophages. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.