Anti-Adiponectin (ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kD)

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: ELISA (direct and indirect): 1:5000-1:10,000, Immunoprecipitation: 1-5ug/sample in a final reaction volume of 500-1000ul., Western Blot: 1:5000-1:10,000 using ECL, using blocking and dilution buffer TBST, 0.05% Tween 20, 5% skim milk, suggested incubation time is 1 hour at room temperature, Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.