Anti-ADAM8 Ectodomain (a Disintegrin and Metalloproteinase Domain 8, MS2, CD156, CD156 MS2)

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells using human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin., Western Blot: 1ug/ml using recombinant human ADAM8 aa158-653 under non-reducing conditions only. Does not detect recombinant human ADAM8 aa158--497., Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human ADAM8 aa158-653. Does not detect recombinant human ADAM8 aa158-497. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.