Anti-ADAM 12 (MCMP, MLTN, MLTNA, MCMPMltna, Meltrin alpha, A disintegrin and metalloproteinase domai

ADAM12 (A disintegrin and metalloprotease domain 12, also meltrin-alpha) is a , 120kD type I transmembrane glycoprotein member of the metzincin family of , proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that , contains a 25kD proregion (aa 29-207) and a 92kD mature molecule. , Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface , expression. A 68kD soluble splice form exists in pregnancy where 36 aa , replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform , remains associated with its prodomain via noncovalent linkage, possibly acting , as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and , 79% aa identity with bovine and mouse ADAM12, respectively. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 0.1- 0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.