Anti-Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2)

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5). Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. See Control Peptide, A0855-75J1., Immunocytochemistry: 5-10ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from Ramos cells , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.