Anti-5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purin

CD73 (also known as [ecto]-5'-nucleotidase/5'-NT) is a variably glycosylated, 69-73kD member of the 5'-nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3 Treg lymphocytes, FDCs, and B cells. CD73 hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human CD73 is a 523aa GPI-linked protein (aa27-549). It contains a large Zn-dependent nucleotidase catalytic region (aa28-532) and a C-terminal substrate binding site (aa500-506). On the cell surface, it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa405-454, and a second possesses a 12aa substitution for aa253-574. Over aa1-511, human CD73 shares 88% aa identity with both mouse and rat CD73. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, CHO-derived, recombinant human 5' Nucleotidase/CD73. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.