GenieClone DNA Assembly Cloning Kit

GenieClone DNA Assembly Cloning Kit
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
G-MORV0004.25 25 rxns - -

9 - 20 Werktage*

264,00 €
G-MORV0004.50 50 rxns - -

9 - 20 Werktage*

428,00 €
 
GenieClone DNA Assembly Cloning Kit allows for the highly efficient cloning and assembly of 1-5... mehr
Produktinformationen "GenieClone DNA Assembly Cloning Kit"
GenieClone DNA Assembly Cloning Kit allows for the highly efficient cloning and assembly of 1-5 DNA fragments in as little as 15 minutes into any vector at any site. This system utilizes ligation-independent technology and a novel GenieClone recombinase to significantly reduce self-ligating colonies. The enhanced GenieClone Recombinase and highly optimized buffer included in the 2x GenieClone Mix significantly improve the recombination efficiency and the tolerance to impurities. The pCE-ONE vector is compatible with most PCR products, enabling the specific PCR fragments to be used directly for recombination without any pre-treatment. Utilizing a simple procedure, the vector is first linearized at the cloning site. A small overlapping sequence at each end of the cloning site is added to the insert through PCR. The insert and the linearized vector both with overlapping 5'- and 3' (15 bp - 20 bp) sequences are mixed in an appropriate ratio and incubated with GenieClone Recombinase at 50? for 5 - 15 min before transformation of competent cells for successful DNA cloning and assembly.
Hersteller: Assay Genie
Hersteller-Nr: MORV0004

Eigenschaften

Anwendung: Highly efficient cloning

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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