Vitamin D-binding protein (Gc), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 17-476aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-sumostar-tagged. Target Protein Sequence: LERGRDYEKD KVCNELAMLG KEDFRSLSLI LYSRKFSSST FEQVNQLVKE VVSLTEECCA EGADPTCYDT RTSELSVKSC ESDAPFPVHP GTPECCTKEG LERKLCMAAL SHQPQEFPTY VEPTNDEICE AFRRDPKGFA DQFLYEYSSN YGQAPLPLLV AYTKNYLSMV GSCCTSANPT VCFVKERLQM KHLSLLTTMS NRVCSQYAAY GKEKSRLSHL IKLAQKVPTA NLENVLPLAE DFTEILSRCC ESTSEDCMAS ELPEHTIKIC QNLSKKNSKF EECCQENTPM NIFMCTYFMP AAEPLQLPAI KLPTGKDLCG QSTTQAMDQY TFELSRRTQV PEVFLSKVLE PTLKTLRECC DTQDSVACFS TQSPLLKRQL TSFIEKGQEM CADYSENTFT EYKKKLAERL RTKTPNTSPA ELKDMVEKHS DFASKCCSIN SPPLYCSSQI DAEMIDTLQS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. Reference: "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007). Function: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.