Thymic stromal lymphopoietin (TSLP) (R127A,R130S) (Active), recombinant Macaca fascicularis

Organism: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey). Source: Mammalian cell. Expression Region: 29-159aa(R127A,R130S). Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: YDFTNCDFQK IEADYLRTIS KDLITYMSGT KSTDFNNTVS CSNRPHCLTE IQSLTFNPTP RCASLAKEMF ARKTKATLAL WCPGYSETQI NATQAMKKAR KSKVTTNKCL EQVSQLLGLW RRFIRTLLKK Q. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Loaded Cynomolgus TSLP(R127A, R130S) on 96-Flat plate, can bind anti-TSLP antibody(CSB-RA025141MA1HU), with an affinity constant of 4.41 nM as determined in BLI assay (Gator Prime). Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: Warren W., Wilson R.K. Submitted to EMBL/GenBank/DDBJ databases (MAR-2013). Function: nan