SCF (human), animal-free recombinant

Product Description: SCF, a hematopoietic growth factor, functions by activating the c-Kit receptor. This interaction is crucial for the survival, proliferation, and differentiation of hematopoietic cells committed to melanocyte and germ cell lineages. Interestingly, Human SCF has limited activity on murine cells, whereas murine and rat SCF are fully functional on Human cells. The Human SCF gene encodes a 273 amino acid transmembrane protein, consisting of a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The soluble form of SCF is produced by proteolytic cleavage of the membrane-anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide comprising 165 amino acid residues, representing the sequence of the secreted soluble form of SCF. . Amino acid sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA. Endotoxin concentration: <0.1 EU/µg. Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is = 2.0 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg. Contains no preservative. Protein function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. [The UniProt Consortium]