Saxiphilin, partial, Lithobates catesbeiana, recombinant

Organism: American bullfrog. Source: Yeast. Expression Region: 484-844aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AHLPSKNKVR WCTINKLEKM KCDDWSAVSG GAIACTEASC PKGCVKQILK GEADAVKLEV QYMYEALMCG LLPAVEEYHN KDDFGPCKTP GSPYTDFGTL RAVALVKKSN KDINWNNIKG KKSCHTGVGD IAGWVIPVSL IRRQNDNSDI DSFFGESCAP GSDTKSNLCK LCIGDPKNSA ANTKCSLSDK EAYYGNQGAF RCLVEKGDVA FVPHTVVFEN TDGKNPAVWA KNLKSEDFEL LCLDGSRAPV SNYKSCKLSG IPPPAIVTRE ESISDVVRIV ANQQSLYGRK GFEKDMFQLF SSNKGNNLLF NDNTQCLITF DRQPKDIMED YFGKPYYTTV YGASRSAMSS ELISACTIKH C. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds specifically to the neurotoxin saxitoxin. Its physiological role may be to transport or sequester an endogenous organic molecule other than Fe3+. It may participate in a detoxification mechanism for neutralizing a microbial toxin. Reference: "Purification and partial sequencing of saxiphilin, a saxitoxin-binding protein from the bullfrog, reveals homology to transferrin."Li Y., Moczydlowski E.J. Biol. Chem. 266:15481-15487(1991). Function: Binds specifically to the neurotoxin saxitoxin. Its physiological role may be to transport or sequester an endogenous organic molecule other than Fe(3+). It may participate in a detoxification mechanism for neutralizing a microbial toxin.