Protein Wnt-4 (Wnt4), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 23-351aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SNWLYLAKLS SVGSISEEET CEKLKGLIQR QVQMCKRNLE VMDSVRRGAQ LAIEECQYQF RNRRWNCSTL DSLPVFGKVV TQGTREAAFV YAISSAGVAF AVTRACSSGE LEKCGCDRTV HGVSPQGFQW SGCSDNIAYG VAFSQSFVDV RERSKGASSS RALMNLHNNE AGRKAILTHM RVECKCHGVS GSCEVKTCWR AVPPFRQVGH ALKEKFDGAT EVEPRRVGSS RALVPRNAQF KPHTDEDLVY LEPSPDFCEQ DIRSGVLGTR GRTCNKTSKA IDGCELLCCG RGFHTAQVEL AERCGCRFHW CCFVKCRQCQ RLVEMHTCR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung . Required for normal mesenchyme to epithelium transition during embryonic kidney development . Required for the formation of early epithelial renal vesicles during kidney development . Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries . Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary . Required for normal lung development and for normal patterning of trachael cartilage rings . Reference: "Generation of an allele to inactivate Wnt4 gene function conditionally in the mouse." Shan J., Jokela T., Peltoketo H., Vainio S. Genesis 47:782-788(2009). Function: nan