Protein Wnt-2 (WNT2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-360aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SWWYMRATGG SSRVMCDNVP GLVSSQRQLC HRHPDVMRAI SQGVAEWTAE CQHQFRQHRW NCNTLDRDHS LFGRVLLRSS RESAFVYAIS SAGVVFAITR ACSQGEVKSC SCDPKKMGSA KDSKGIFDWG GCSDNIDYGI KFARAFVDAK ERKGKDARAL MNLHNNRAGR KAVKRFLKQE CKCHGVSGSC TLRTCWLAMA DFRKTGDYLW RKYNGAIQVV MNQDGTGFTV ANERFKKPTK NDLVYFENSP DYCIRDREAG SLGTAGRVCN LTSRGMDSCE VMCCGRGYDT SHVTRMTKCG CKFHWCCAVR CQDCLEALDV HTCKAPKNAD WTTAT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Reference: "Evidence supporting WNT2 as an autism susceptibility gene." Wassink T.H., Piven J., Vieland V.J., Huang J., Swiderski R.E., Pietila J., Braun T., Beck G., Folstein S.E., Haines J.L., Sheffield V.C. Am. J. Med. Genet. 105:406-413(2001). Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.