Probetacellulin, cleaved to Betacellulin, active (BTC), Recombinant, Human

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. {ECO:0000269, PubMed:8570211}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05ng/ml, corresponding to a specific activity of ? 2.0x10e7 IU/mg. Sequence: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY, Molecular Weight: 9.0kD, PubMed ID: 8439318, 15489334, 8144591, 8919026, 8570211, 10867024, 12074582, Gene Name: BTC, Swiss Prot: P35070, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.