Netrin-1 (Ntn1), recombinant mouse

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 25-604aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: GPGLSMFAGQ AAQPDPCSDE NGHPRRCIPD FVNAAFGKDV RVSSTCGRPP ARYCVVSERG EERLRSCHLC NSSDPKKAHP PAFLTDLNNP HNLTCWQSEN YLQFPHNVTL TLSLGKKFEV TYVSLQFCSP RPESMAIYKS MDYGRTWVPF QFYSTQCRKM YNRPHRAPIT KQNEQEAVCT DSHTDMRPLS GGLIAFSTLD GRPSAHDFDN SPVLQDWVTA TDIRVAFSRL HTFGDENEDD SELARDSYYY AVSDLQVGGR CKCNGHAARC VRDRDDSLVC DCRHNTAGPE CDRCKPFHYD RPWQRATARE ANECVACNCN LHARRCRFNM ELYKLSGRKS GGVCLNCRHN TAGRHCHYCK EGFYRDMGKP ITHRKACKAC DCHPVGAAGK TCNQTTGQCP CKDGVTGITC NRCAKGYQQS RSPIAPCIKI PVAPPTTAAS SVEEPEDCDS YCKASKGKLK MNMKKYCRKD YAVQIHILKA DKAGDWWKFT VNIISVYKQG TSRIRRGDQS LWIRSRDIAC KCPKIKPLKK YLLLGNAEDS PDQSGIVADK SSLVIQWRDT WARRLRKFQQ REKKGKCKKA. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: The immunization strategy should be optimized (antigen dose, regimen and adjuvant). Relevance: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Involved in dorsal root ganglion axon projection towards the spinal cord . It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Reference: n/a. Function: nan