Multifunctional fusion protein (Il1b), Mesocricetus auratus, recombinant

Organism: Mesocricetus auratus (Golden hamster). Source: E.coli. Expression Region: 1-267aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged and C-terminal Strep II-tagged. Target Protein Sequence: MATVPELDSE MIAFHSDEND LFFEVDGLQK MKSCFQSLDL SYPDESIQLQ ISKQDLNKSF RQVVSVIVAV EKLWNTPVPC PWTFQDEDLR TFFSFIFEEE PIFCDSWDGE LIVADAPIRQ LHCRLRDEQQ KCLVLSDPCE LKALHLNGQN INQQVVFSMS FVQGETSNNK IPVALGLKGK NLYLSCVMKG DTPTLQLESV DPKQYPKKKM EKRFVFNKIE VKTKVEFESA QFPNWYISTS QAEHKPVFLG NNSGQDLVDF TMESVSS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Reference: 1 RefSeq Submitted (APR-2022) to UniProtKB Cited for: IDENTIFICATION. Function: nan