Interleukin-36 Gamma, mouse recombinant (rmIL-36G)

IL36G mouse recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.3 kDa. IL-36 gamma belongs to the IL-1 family which includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36 Ra (IL-1F5), IL-36a (IL-1F6), IL-36b (IL-1F8), IL-37 (IL-1F7) and IL-1F10. ). The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. IL-36g is an 18-22 kDa, 169 amino acids intracellular and secreted protein which holds no signal sequence, no prosegment and no potential N-linked glycosylation sites. human IL-36g shares 58%- 69% amino acid sequence homology with mouse, rat, bovine and equine IL-36g, and 23 - 57% amino acid sequence homology with other family members. The IL-36g receptor is a mixture of IL-1 Rrp2, mostly located in epithelia and keratinocytes, and the extensively expressed IL-1 RAcP. All IL-36 (a, b and g) activate N F-?B and MAPK pathways in an IL-1 Rrp2 dependent reaction. Additionally, IL-36g induces production of inflammatory cytokines and chemokines like CXCL8/IL-8. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.