Interleukin-20 protein (IL20) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.Coli. Expression Region: 25-176aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: M+LKTLNLGSCV IATNLQEIRN GFSEIRGSVQ AKDGNIDIRI LRRTESLQDT KPANRCCLLR HLLRLYLDRV FKNYQTPDHY TLRKISSLAN SFLTIKKDLR LCHAHMTCHC GEEAMKKYSQ ILSHFEKLEP QAAVVKALGE LDILLQWMEE TE. Purity: >95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Ralpha and human IL-20Rbeta co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 ×106 IU/mg. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.2, with trehalose. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. {ECO:0000269, PubMed:16511554}. Reference: n/a. Function: Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3.