Interleukin-17A (CHO-expressed), Recombinant, Mouse, aa26-158 (IL-17A, CTLA-8)

Interleukin-17A (IL-17A), also known as CTLA­8, is a 15­20kD glycosylated cytokine that plays an important role in anti­microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL­17A shares 61% and 89% amino acid sequence identity with human and rat IL­17A, respectively (3, 4). IL­17A is secreted by Th17 cells, ga,ma/delta T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide­linked homodimers as well as disulfide­linked heterodimers with IL­17F (5, 6). IL­17A exerts its effects through the transmembrane IL­17 RA in complex with IL­17 RC or IL­17 RD (7, 8). Both IL­17 RA and IL­17 RC are required for responsiveness to heterodimeric IL­17A/F (7). IL­17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9­12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9­11). IL­17A/F likewise induces neutrophil migration, but IL­17F does not (11). IL­17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF­alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL­17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL­17A has been shown to exert either tumorigenic or anti­tumor effects (17, 18). Source: Recombinant protein corresponding to aa26-158 from mouse IL-17A, CHO-derived. Molecular Weight: ~15-22kD, Endotoxin: <0.01EU/1ug (LAL method), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.