Il33, Recombinant, Mouse (Interleukin-33, IL-33)

IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Recombinant mouse IL-33 is a 17.5kD protein containing 158aa. Source: Recombinant protein corresponding to mouse IL-33, expressed in E.coli. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 was found to be 0.5ng/ml. Specific Activity: 2x10e6units/mg. Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.