IL33, Recombinant, Human (Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Fa

Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Recombinant human IL-33 is a 17.9kD protein containing 159aa. Source: Recombinant protein corresponding to human IL-33, expressed in E.coli. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine D10S cells. The ED50 was found to be 0.05ng/ml, Specific Activity: 2x10e7units/mg, Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.