Il13, Recombinant, Rat (Interleukin-13, IL-13, T-cell Activation Protein P600, Il-13)

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is a 12.3kD protein consisting of 113aa. Source: Recombinant protein corresponding to rat IL-13, expressed in E.coli. Biological Activity: Determined by the dose-dependent proliferation of TF-1 cells. The ED50 was found to be <5ng/ml. Specific Activity: >2x10e5units/mg. Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSIVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.