Glial Fibrillary Acidic Protein (GFAP) Recombinant, Human

Recombinant protein corresponding to aa254-374 with two N-terminal Tags, His-Tag and S-Tag from human GFAP, expressed in E. coli (P14136), Amino Acid Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-EEWYRSKF ADLTDAAARN AELLRQAKHE ANDYRRQLQS LTCDLESLRG TNESLERQMR EQEERHVREA ASYQEALARL EEEGQSLKDE MARHLQEYQD LLNVKLALDI EIATYRKLLE GEE, Predicted Molecular Weight: 20kD, Accurate Molecular Weight: 25kD as determined by SDS-PAGE reducing conditions, Note: The possible reasons that the actual band size differs from the predicted are as follows:, 1. Splice variants: Alternative splicing may create different sized proteins from the same gene, 2. Relative charge: The composition of amino acids may affect the charge of the protein., 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc., 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form., 5. Polymerization of the target protein: Dimerization, multimerization etc. Predicted Isoelectric Point: 5.1, Subcellular Location: Cytoplasm, Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE., Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed., MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD , Double intensity: 10kD, 18kD, 26kD, Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels, 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.