Erythroferrone (Erfe), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 25-340aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: LGVPESAEPV GTHARPQPPG AELPAPPANS PPEPTIAHAH SVDPRDAWML FVKQSDKGIN SKRRSKARRL KLGLPGPPGP PGPQGPPGPF IPSEVLLKEF QLLLKGAVRQ RESHLEHCTR DLTTPASGSP SRVPAAQELD SQDPGALLAL LAATLAQGPR APRVEAAFHC RLRRDVQVDR RALHELGIYY LPEVEGAFHR GPGLNLTSGQ YTAPVAGFYA LAATLHVALT EQPRKGPTRP RDRLRLLICI QSLCQHNASL ETVMGLENSS ELFTISVNGV LYLQAGHYTS VFLDNASGSS LTVRSGSHFS AILLGL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake. Reference: "Myonectin (CTRP15), a novel myokine that links skeletal muscle to systemic lipid homeostasis." Seldin M.M., Peterson J.M., Byerly M.S., Wei Z., Wong G.W. J. Biol. Chem. 287:11968-11980(2012). Function: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage