Trifunctional enzyme subunit alpha, mitochondrial (HADHA), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 37-763aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: TRTHINYGVK GDVAVVRINS PNSKVNTLSK ELHSEFSEVM NEIWASDQIR SAVLISSKPG CFIAGADINM LAACKTLQEV TQLSQEAQRI VEKLEKSTKP IVAAINGSCL GGGLEVAISC QYRIATKDRK TVLGTPEVLL GALPGAGGTQ RLPKMVGVPA ALDMMLTGRS IRADRAKKMG LVDQLVEPLG PGLKPPEERT IEYLEEVAIT FAKGLADKKI SPKRDKGLVE KLTAYAMTIP FVRQQVYKKV EEKVRKQTKG LYPAPLKIID VVKTGIEQGS DAGYLCESQK FGELVMTKES KALMGLYHGQ VLCKKNKFGA PQKDVKHLAI LGAGLMGAGI AQVSVDKGLK TILKDATLTA LDRGQQQVFK GLNDKVKKKA LTSFERDSIF SNLTGQLDYQ GFEKADMVIE AVFEDLSLKH RVLKEVEAVI PDHCIFASNT SALPISEIAA VSKRPEKVIG MHYFSPVDKM QLLEIITTEK TSKDTSASAV AVGLKQGKVI IVVKDGPGFY TTRCLAPMMS EVIRILQEGV DPKKLDSLTT SFGFPVGAAT LVDEVGVDVA KHVAEDLGKV FGERFGGGNP ELLTQMVSKG FLGRKSGKGF YIYQEGVKRK DLNSDMDSIL ASLKLPPKSE VSSDEDIQFR LVTRFVNEAV MCLQEGILAT PAEGDIGAVF GLGFPPCLGG PFRFVDLYGA QKIVDRLKKY EAAYGKQFTP CQLLADHANS PNKKFYQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity. It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP. Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity. Reference: n/a. Function: nan