Transformer-2 protein homolog beta (TRA2B), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 2-288aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SDSGEQNYGE RESRSASRSG SAHGSGKSAR HTPARSRSKE DSRRSRSKSR SRSESRSRSR RSSRRHYTRS RSRSRSHRRS RSRSYSRDYR RRHSHSHSPM STRRRHVGNR ANPDPNCCLG VFGLSLYTTE RDLREVFSKY GPIADVSIVY DQQSRRSRGF AFVYFENVDD AKEAKERANG MELDGRRIRV DFSITKRPHT PTPGIYMGRP TYGSSRRRDY YDRGYDRGYD DRDYYSRSYR GGGGGGGGWR AAQDRDQIYR RRSPSPYYSR GGYRSRSRSR SYSPRRY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA. Reference: "Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing." Tacke R., Tohyama M., Ogawa S., Manley J.L. Cell 93:139-148(1998). Function: nan