Transcobalamin-1 (TCN1), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 24-433aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EICEVSEENY IRLKPLLNTM IQSNYNRGTS AVNVVLSLKL VGIQIQTLMQ KMIQQIKYNV KSRLSDVSSG ELALIILALG VCRNAEENLI YDYHLIDKLE NKFQAEIENM EAHNGTPLTN YYQLSLDVLA LCLFNGNYST AEVVNHFTPE NKNYYFGSQF SVDTGAMAVL ALTCVKKSLI NGQIKADEGS LKNISIYTKS LVEKILSEKK ENGLIGNTFS TGEAMQALFV SSDYYNENDW NCQQTLNTVL TEISQGAFSN PNAAAQVLPA LMGKTFLDIN KDSSCVSASG NFNISADEPI TVTPPDSQSY ISVNYSVRIN ETYFTNVTVL NGSVFLSVME KAQKMNDTIF GFTMEERSWG PYITCIQGLC ANNNDRTYWE LLSGGEPLSQ GAGSYVVRNG ENLEVRWSKY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds vitamin B12 with ftomolar affinity and protects it from the acidic environment of the stomach. Reference: Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein.Johnston J., Bollekens J., Allen R.H., Berliner N.J. Biol. Chem. 264:15754-15757(1989). Function: Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.