Thrombin (human)

Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin and potentiates the procoagulation process by activating factors V, VIII, XI, and XIII. Conversely, thrombin aids the anticoagulant process by activating protein C. Activated protein C inhibits activated factors V and VIII ultimately down-regulating further thrombin production. Thrombin is often used as a potent platelet activator in vitro. Platelet responses to thrombin are mediated through protease-activated receptors (PARs). Thrombin-mediated signaling regulates a diverse range of metabolic processes including activation of phospholipases in platelets, endothelial cells, and cardiac myocytes.Synonyms: Activated Factor II [IIa]. Source: Human blood, all source material tested and found non-reactive to HTLV-III and HBsAg using FDA approved assay methods. MW: 38 kDa. Formulation: (Request formulation change), 5 mM sodium citrate, pH 6.5, containing 0.01% PEG and 20 mM sodium chloride. Specific Activity: > 2,000 NIH units/mg.